Isolation and characterization of psychrophiles producing cold-active b-galactosidase
نویسندگان
چکیده
T. NAKAGAWA, Y. FUJ IMOTO, M. UCHINO, T . MIYAJ I , K . TAKANO AND N. TOMIZUKA. 2003. Aims: The present study was conducted to screen for psychrophilic micro-organisms that are able to hydrolyse lactose at low temperature, and to examine the cold-active b-galactosidase produced by the isolated psychrophilic micro-organisms. Methods and Results: Psychrophilic bacteria, which grow on lactose as a sole carbon source, were isolated from soil from Hokkaido, Japan. The phenotype and sequence of 16S rDNA of the isolated strains indicated a taxonomic affiliation to Arthrobacter psychrolactophilus. The isolated A. psychrolactophilus strains were able to grow on lactose at below 5 C, and showed cold-active b-galactosidase activity, which was highly specific at even 0 C. Conclusions: Facts in this study may indicate the possibility that the isolated strains produce novel b-galactosidases that are able to hydrolyse lactose at low temperature, although some strains have isozymes. Significance and Impact of the Study: It may be possible that the cold active b-galactosidases from the isolated strains can be applied to the food industry, e.g. processing of milk and whey below 5 C.
منابع مشابه
Structural prediction and comparative docking studies of psychrophilic β- Galactosidase with lactose, ONPG and PNPG against its counter parts of mesophilic and thermophilic enzymes
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